01915nam a2200241 4500 NALT 20251224113502.0 251105b th ||||| |||| 00| 0 eng d NALT QR 189.5.R3 A64D 2023 Apidsada Chorpunkul. 66885 The development of therapeutic monoclonal antibodies against rabies virus from immunized phage-displayed libraries / Apidsada Chorpunkul. Nakhonpathom : Mahidol University, 2023. 276 p. : ill., tables, chart ; 30 cm. Chapter 1 Introduction and objectives -- Chapter 2 Literature reviews -- Chapter 3 Materials and methods -- 3.1 Sample collection and Preparation -- 3.2 Plasma RFFIT assay and PBMC isolation -- 3.3 Total RNA extraction and cDNA synthesis -- 3.4 Variable heavy (VH) and light chain (VL, kappa and lambda) fragment amplification -- 3.5 Generation of single chain variable fragments (scFv) -- 3.6 Construction of scFv libraries -- 3.7 The scFv phage display library construction -- 3.8 Biopanning (Affinity selection) -- 3.9 Specific binding ELISA of individual clones -- 3.10 DNA sequencing and CDR determination -- 3.11 Soluble scFv expression -- 3.12 Purification of soluble scFv antibodies -- 3.13 Modified Rapid Fluorescent Foci Inhibition Test (RFFIT): neutralization assay -- 3.14 Sensitivity test and binding ability of neutralizing scFv clones by dot-blot ELISA -- 3.15 Bioinformatics -- Chapter 4 Results -- 4.1 Human therapeutic monoclonal antibodies against RABV -- 4.2 Caine therapeutic monoclonal antibodies against RABV -- Chapter 5 Discussion -- Chapter 6 Conclusion. Rabies 66905 Rabies virus 66904 lcc RSH jidapa.t ACQSTF 2025-11-05 varee.k LIBSTF 2025-11-06 jamriang.p CATSTF 2025-11-06 107428 107428 1020 นางจำเรียง ระวังสำโรง 0 0 lcc 0 0 CRSH NALT NALT RSH 2025-11-05 0 Res QR 189.5.R3 A64D 2023 3961215852 2025-11-05 00:00:00 2025-11-05 RSH